Investigation of DNA repair in human oocytes and preimplantation embryos
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چکیده
منابع مشابه
O-35: Over-Expression of XRCC1 As Potential Biomarker for Poor Prognosis in Human Preimplantation Embryos: Selection by Study of 84 Genes Involved in DNA Damage Signaling Pathways
Background: Chromosome abnormalities are associated with poor morphology and development in human preimplantation embryos, all together lead to poor outcomes. This study aimed to explore altered expression of DNA damage pathways in “poor morphological and development embryos with sever aneuploidies”. Materials and Methods: Surplus day-4 embryos of PGD cases were pooled in two groups: Poor progn...
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Purpose: The aim of this study was to assay the profile of MCT1 & MCD in mouse unfertilized & fertilized oocytes and preimplantation embryos In vivo and In vitro. Materials and Methods: The presence of mRNAs encoding MCT1 & MCD3 were determined On unfertilized and fertilized oocytes, 2-cell, morulae, blastocyst and cultured embryos in plus glucose KSOM, minus glucose KSOM and pulse glucose KSO...
متن کاملExpression of genes regulating chromosome segregation, the cell cycle and apoptosis during human preimplantation development.
BACKGROUND Appropriate gene expression is vital for the regulation of developmental processes. Despite this fact there is a remarkable paucity of information concerning gene activity during preimplantation development. METHODS We employed reverse transcription and real-time fluorescent PCR to quantify the expression of nine genes (BRCA1, BRCA2, ATM, TP53, RB1, MAD2, BUB1, APC and beta-actin) ...
متن کاملP-31: Effect of Cryptop Vitrification on DNAMethylation Pattern of Oct4 and Mest Genes inMurine Preimplantation Embryos
Background: Because of the protection of surplus embryos, Cryopreservation is usually used in ART. It is not clear, the vitrified-warmed embryos that have normal morphology, be normal in genetic level, too. DNA methylation of gene regulatory regions can causes inhibition of gene expression. We study effect of vitrification method of cryopreservation on DNA methylation and gene expression level ...
متن کاملSmoking and reproduction: gene damage to human gametes and embryos.
Assisted conception is a useful methodology for detecting disturbances in clinical outcome, meiotic maturation, and genetic integrity of human gametes. Germinal cells are vulnerable to genetic damage from smoking, but can repair damage during meiosis. In ejaculated spermatozoa, repair capacity declines drastically. Smoking alters the meiotic spindle of oocytes and spermatozoa, leading to chromo...
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تاریخ انتشار 2010